Purpose
The purpose of this
document is to provide standard operating procedures for the use of autoclaves.
Autoclaving is a process used to destroy microorganisms and decontaminate
biohazardous waste and microbiological equipment used at Biosafety Level 1, 2,
3 and 4.
Scope
Autoclaves are widely
used in microbiology, medicine, Podiatry, tattooing, body piercing, veterinary
science, mycology, dentistry, and prosthetics fabrication. They vary in size
and function depending on the media to be sterilized. Typical loads include
laboratory glassware, other equipment and waste, surgical instruments and
medical waste.
A
notable growing application of autoclaves is the pre-disposal treatment and
sterilization of waste material, such as pathogenic hospital waste. Machines in
this category largely operate under the same principles as conventional
autoclaves in that they are able to neutralize potentially infectious agents by
utilizing pressurized steam and superheated water. A new generation of waste
converters is capable of achieving the same effect without a pressure vessel to
sterilize culture media, rubber material, gowns, dressing, gloves, etc. It is
particularly useful for materials which cannot withstand the higher temperature
of a hot air oven.
Responsibility
- To organize the Job training program for implementation
of SOP.
- To monitor overall activities in adherence to the
compliance of approved SOP.
- To arrange necessary servicing and
maintenance.
- To check the recorded documents for its correctness,
accuracy and completeness.
- To operate the instrument as per SOP.
- To record the observations as per SOP.
- To maintain the documents as per SOP
Introduction
An autoclave is a device used to sterilize
equipment and supplies by subjecting them to high pressure saturated steam at
121 °C for around 15–20 minutes depending on the size of the load and the
contents. It was invented by Charles Chamberland in 1879, although a precursor
known as the steam digester was created by Denis Papin in 1679. The name comes
from Greek auto-, ultimately meaning self, and Latin clavis meaning key—a
self-locking device.
Materials
And Procedure
A.
Material
-
- Media
- Erlenmeyer
- Cotton
- Autoclave
B.
Procedures
1.)
First, The materials to be sterilized,
stored in a erlenmeyerl corked with cotton and wrapped in newspaper
2.)
Second, Autoclave filled with distilled
water up to a limit of autoclave equipment filter
3.)
Next, Materials are inserted into the
autoclave, and then closed, locking autoclave rotate in the same direction and
balanced then the air valve is closed.
4.)
Third, Heated up to 10 lbs/in2
air pressure, after which the valve in the open air until the air pressure pada
0 lbs/in2
5.)
Then, Air valve is closed and the air
pressure allowed to rise back up to 15 lbs/in2 (1 atm) and a temperature of 121
0C (250 0F) and then left for 15 minutes
6.)
Next, Heating or power is turned off and
the autoclave air valve opened slowly in order to plug in the autoclave lid can
not be separated.
7.)
Finish, After cold ingredients were
taken and stored in a closet.
Reference
www.asu.edu/uagc/EHS/documents/autoclave-sop.pdf
PraktikumMikrobiologi. 2011. Pg 44. Fatmawaty, ST,
dkk, Smk SMAK Makassar. Makassar
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