BHS.INGGRIS "SOP AAS'

·         Purpose

Structured to give you an idea of how the operation of AAS novA 300 correctly

·         Scope

Atomic absorption spectroscopy (AAS) is a spectroanalytical procedure for the qualitative and quantitative determination of chemical elements employing the absorption of optical radiation (light) by free atoms in the gaseous state. In analytical chemistry the technique is used for determining the concentration of a particular element (the analyte) in a sample to be analyzed. AAS can be used to determine over 70 different elements in solution or directly in solid samples.

·         Responsibilities

It is the responsibility of designated personnel in Research lab to train  staff and students on this procedure and to ensure adherence to this  procedure.

·         Introduction

The Standard Operating Procedures (SOPs) for instruments, include  the standard protocol to be followed for the proper and efficient functioning of instruments during the operation by students, faculty, lab technicians and other researchers of the university / college. 

·         Procedure

1.      Open valve / gas regulator used

2.      Air compressor valve (located below) and compressor shut in on it

3.      Instruments on it (there is a button on the right of the green color tool)

4.      Wait until the display "AAS novA 300" appears.

5.      Click "HCl TURRED", place the position of the arrow on the position of the lights (if you want to replace the lamp select "CHANGE" and select the elements that would be tested) and then click OK - OK to the start menu

6.      Click "METHOD DEVELOPMENT" click OK

7.      Click "SPECTROPHOTOMETER", "OPTICAL PARAMETER" make sure that is analyzed in an active state, click the "ENERGY" or "GAJN" and make sure the energy around 60-80 then ok

8.      Click "CALIBRATION", "CALIBRATION STANDARD" click "CONDITION" notice "NO.OFF SATNDARD" adjust the amount of the standard to be analyzed. Click the "Table", "CLEAR THE TABLE", click "CAL-STANO". 1. Click "CHANGE" select "CONCENTRATION" if there is already a number, "DELETE", and then replace it with a new number. "ENTER", the same thing is done for the other standards. Click OK again

9.      Click "SAMPLES" select lines enabling filled replace the sample data by clicking "CHANGE" enter the name of the sample you click OK then OK again.

10.        Blower in on it

11.        Click "FLAME", click on "TEST THE WATER" notice must be aerosolized form (only appears when a new tool is operated) TEST END.

12.        To light the burner, click "EGNITE FLAME", wait until the burner flame appears, click OK.

13.        Click "CALIBRATION" and "STANDARD CALIBRATION", "TABLE", select "START / ABS", OK.

14.        Follow the instructions on the screen. Click OK.
"ALWAYS REMEMBER AQUADEST WILL REPLACE THE ENTIRE SOLUTION"

15.        Click "SAMPLES", select the name of the sample that you want to measure, select the "LINE" and "SAMPLE RUN" click OK until the instruction the monitor. Follow the instructions to complete.

16.        Click OK, then OK again until the start menu

17.        Click "FLAME" and "EXTINGUISH", click OK

18.        When done click "EXIT", then the instrument is turned off by pressing the right tools.

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